Dieses Anti-Nestin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Nestin in IF und IHC (p). Geeignet für Human und Maus. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.
NES
Reaktivität: Human
WB, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
IF: 1:10~50. IHC-P: 1:50~100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Chen, Ou, Song, Xian, Ouyang, Xie, Xue, Sun: "Generation of integration-free induced pluripotent stem cells (GZHMUi001-A) by reprogramming peripheral blood mononuclear cells from a 47, XXX syndrome patient." in: Stem cell research, Vol. 23, pp. 57-60, (2018) (PubMed).
May, Sureban, Lightfoot, Hoskins, Brackett, Postier, Ramanujam, Rao, Wyche, Anant, Houchen: "Identification of a novel putative pancreatic stem/progenitor cell marker DCAMKL-1 in normal mouse pancreas." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 2, pp. G303-10, (2010) (PubMed).
Target
Nestin (NES)
Andere Bezeichnung
Nestin
Hintergrund
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.